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To perform bisulfite sequencing non methylated Cytosines are converted from Cytosine to Uracil in attempts to be able to differentiate between the methylated and non methylated Cytosines. This is accomplished through a series of chemical reactions that involve adding and then removing sulfur containing groups from non methylated Cytosines, converting non methylated Cytosines to Uracil. Once complete, the targeted segment of DNA is amplified and then sequenced.