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The siRNA sequences targeting GL2 (Acc. No. 21),lid daidaihua, whereby 50 and 501 bp hG dsRNA corresponded to positions 121 and 121 respectively, to the start codon.For annealing of siRNAs, 20 M single strands were incubated in annealing buffer (100 mM potassium acetate, 30 mM HEPES KOH at pH 7.4,50 boxes of meizitang gel and 50 boxes o, 2 mM magnesium acetate) for 1 min at 90 followed by 1 h at 37 The 37 incubation step was extended overnight for the 50 and 500 bp dsRNAs,fruta planta original site, and these annealing reactions were performed at 8.4 M and 0.84 M strand concentrations, respectively.Cell culture S2 cells were propagated in Schneider’s Drosophila medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS) 100 units ml 1 penicillin, and 100 g ml 1 streptomycin at 25 293, NIH/3T3, HeLa S3, HeLa SS6, COS 7 cells were grown at 37 in Dulbecco’s modified Eagle’s medium supplemented with 10% FBS, 100 units ml 1 penicillin, and 100 g ml 1 streptomycin.